The HAD superfamily contains many members that are promiscuous “house-keeping” enzymes. Having no single substrate (i.e. multi-functional) complicates functional analysis and is an example of a caveat that the EFI must address to develop a robust sequence-based strategy. This paper from the EFI HAD Bridging Project examines the structure-function relationship between two such promiscuous enzymes, BT1666 and BT4131 from B. thetaiotaomicron, to order to understand the principles that govern differing specificity in similar enzymes.
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