enzymefunction.org

The Enzyme Function Initiative (EFI) is developing a robust sequence / structure based strategy for facilitating discovery of in vitro enzymatic and in vivo metabolic / physiological functions of unknown enzymes discovered in genome projects. More »

Strategy

 

INTEGRATED STRATEGY

Determining the functions of enzymes discovered through genome sequencing is a major challenge in contemporary biology.  The EFI is using a multi-disciplinary superfamily-based approach for functional assignment that will be applicable to enzymes of all classes.  More »
 

 

 

SEQUENCE
Using bioinformatic analyses, superfamily member sequences (colored circles) are clustered into discrete groups and visualized in sequence similarity networks. Clusters are evaluated for unknown functions to aid selection of targets for the EFI pipeline.  More »

 

STRUCTURE

Structures for targeted enzymes are obtained either by x-ray crystallography or homology modeling.  The structures serve as templates for docking of in silico libraries during virtual screening and enable functional predictions to guide activity screening.  More »

 

REACTION

A variety of in vitro biochemical assays are used to test the computational predictions generated for EFI targets.  Results are used to guide further experiments, such co-crystallization with ligands, and to evaluate the computational methods being developed.  More »

 

FUNCTION

A combination of thorough in vitro analysis and in vivo approaches such as genetic, phenotypic, and metabolomic analysis (as available) cumulates in assigned functions for characterized EFI targets.  Annotations are transferred to receptive public databases.  More »

Announcements

  Signup for our e-mail alert!   |   Download our Newsletters!


New EFI-EST - A web-based sequence similarity network tool from the EFI. More »


New Resource - View the list of organisims investigated by the Microbiology Core. More »


New Dockovalent - The Computation Core releases new docking tool.   More »


New Publications - 8 EFI publications added for 2015.   More »

Publications

2011 Stourman 04

2011 Publication from Stourman, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2011 Stourman 05

2011 Publication from Stourman, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2011 Stourman 06

2011 Publication from Stourman, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2011 Stourman 07

2011 Publication from Stourman, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2011 Stourman 09

2011 Publication from Stourman, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2011 Wu sm 02

2011 Publication from Wu, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2011-Wu-sm-03

2011 Publication from Wu, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2011 Wu 04

2011 Publication from Wu, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2011 Goble 02

2011 Publication from Goble, et al. in ACS Chemical Biology Reprinted with permission. © American Chemical Society

2011 Goble 04

2011 Publication from Goble, et al. in ACS Chemical Biology Reprinted with permission. © American Chemical Society

2011-Huang-03

2011 Publication from Huang, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2011-Huang-04

2011 Publication from Huang, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2011-Huang-05

2011 Publication from Huang, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2011-Huang-06

2011 Publication from Huang, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2011-Huang-07

2011 Publication from Huang, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2011-Huang-08

2011 Publication from Huang, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2011-Huang-09

2011 Publication from Huang, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2011-Huang-09

2011 Publication from Huang, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2011-Huang-10

2011 Publication from Huang, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2011-Carlsson-01

2011 Publication from Carlsson, et al. in Nature Chemical Biology Reprinted with permission. © Macmillan Publishers LTD

2011-Carlsson-02

2011 Publication from Carlsson, et al. in Nature Chemical Biology Reprinted with permission. © Macmillan Publishers LTD

2011-Carlsson-03

2011 Publication from Carlsson, et al. in Nature Chemical Biology Reprinted with permission. © Macmillan Publishers LTD

2011-Carlsson-04

2011 Publication from Carlsson, et al. in Nature Chemical Biology Reprinted with permission. © Macmillan Publishers LTD

2011-Schlessinger-01

2011 Publication from Schlessinger, et al. in Proc Natl Acad Sci USA Reprinted with permission. © National Academy of Sciences, USA

2011-Schlessinger-02

2011 Publication from Schlessinger, et al. in Proc Natl Acad Sci USA Reprinted with permission. © National Academy of Sciences, USA

2011-Schlessinger-03

2011 Publication from Schlessinger, et al. in Proc Natl Acad Sci USA Reprinted with permission. © National Academy of Sciences, USA

2011-Schlessinger-04

2011 Publication from Schlessinger, et al. in Proc Natl Acad Sci USA Reprinted with permission. © National Academy of Sciences, USA

2011-Gerlt-EFI-03_0.jpg

2011 Publication from Gerlt, et al. in Biochemistry Reprinted with permission. © American Chemical Society.

2011-Gerlt-EFI-04

2011 Publication from Gerlt, et al. in Biochemistry Reprinted with permission. © American Chemical Society.

2011-Gerlt-EFI-05

2011 Publication from Gerlt, et al. in Biochemistry Reprinted with permission. © American Chemical Society.

2011-Gerlt-EFI-06.jpg

2011 Publication from Gerlt, et al. in Biochemistry Reprinted with permission. © American Chemical Society.

2011-Gerlt-EFI-07

2011 Publication from Gerlt, et al. in Biochemistry Reprinted with permission. © American Chemical Society.

2011-Fan-02.jpg

2011 Publication from Fan, et al. in Journal of Chemical Information and Modeling Reprinted with permission. © American Chemical Society.

2011-Fan-03.jpg

2011 Publication from Fan, et al. in Journal of Chemical Information and Modeling Reprinted with permission. © American Chemical Society.

2011-Fan-04

2011 Publication from Fan, et al. in Journal of Chemical Information and Modeling Reprinted with permission. © American Chemical Society.

2011-Fan-05

2011 Publication from Fan, et al. in Journal of Chemical Information and Modeling Reprinted with permission. © American Chemical Society.

2011-Fan-06

2011 Publication from Fan, et al. in Journal of Chemical Information and Modeling Reprinted with permission. © American Chemical Society.

2011-Fan-07

2011 Publication from Fan, et al. in Journal of Chemical Information and Modeling Reprinted with permission. © American Chemical Society.

2011-Fan-02.jpg

2011 Publication from Fan, et al. in Journal of Chemical Information and Modeling Reprinted with permission. © American Chemical Society.

2011-Gerlt-Divergent-01

2011 Publication from Gerlt, et al. in Journal of Biological Chemistry Reprinted with permission. © American Society for Biochemistry and Molecular Biology

2011-Gerlt-Divergent-02

2011 Publication from Gerlt, et al. in Journal of Biological Chemistry Reprinted with permission. © American Society for Biochemistry and Molecular Biology

2011-Gerlt-Divergent-03

2011 Publication from Gerlt, et al. in Journal of Biological Chemistry Reprinted with permission. © American Society for Biochemistry and Molecular Biology

2011-Gerlt-Divergent-04

2011 Publication from Gerlt, et al. in Journal of Biological Chemistry Reprinted with permission. © American Society for Biochemistry and Molecular Biology

2011-Brown-02.jpg

2011 Publication from Brown, et al. in Journal of Biological Chemistry Reprinted with permission. © American Society for Biochemistry and Molecular Biology

2011-Brown

2011 Publication from Brown, et al. in Journal of Biological Chemistry Reprinted with permission. © American Society for Biochemistry and Molecular Biology

2011-Brown-03

2011 Publication from Brown, et al. in Journal of Biological Chemistry Reprinted with permission. © American Society for Biochemistry and Molecular Biology

2011-Brown-04.jpg

2011 Publication from Brown, et al. in Journal of Biological Chemistry Reprinted with permission. © American Society for Biochemistry and Molecular Biology

2011-Kamat-01.jpg

2011 Publication from Kamat, et al. in Nature Reprinted with permission. © Macmillan Publishers LTD

2011-Kamat-02.jpg

2011 Publication from Kamat, et al. in Nature Reprinted with permission. © Macmillan Publishers LTD

2011-Kamat-03.jpg

2011 Publication from Kamat, et al. in Nature Reprinted with permission. © Macmillan Publishers LTD

2011-Kamat-04.jpg

2011 Publication from Kamat, et al. in Nature Reprinted with permission. © Macmillan Publishers LTD

Wallrapp-01

2013 Publication from Wallrapp, et al. in Proc Natl Acad Sci USA Reprinted with permission. © National Academy of Sciences, USA

2013 Wallrapp 02

2013 Publication from Wallrapp, et al. in Proc Natl Acad Sci USA Reprinted with permission. © National Academy of Sciences, USA

2013 Wallrapp 03

2013 Publication from Wallrapp, et al. in Proc Natl Acad Sci USA Reprinted with permission. © National Academy of Sciences, USA

2013 wallrapp 04

2013 Publication from Wallrapp, et al. in Proc Natl Acad Sci USA Reprinted with permission. © National Academy of Sciences, USA

2013 wallrapp 05

2013 Publication from Wallrapp, et al. in Proc Natl Acad Sci USA Reprinted with permission. © National Academy of Sciences, USA

2013 Wallrapp 06

2013 Publication from Wallrapp, et al. in Proc Natl Acad Sci USA Reprinted with permission. © National Academy of Sciences, USA

2013 Ghodge

2013 Publication from Ghodge, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Ghodge 02

2013 Publication from Ghodge, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Ghodge 03

2013 Publication from Ghodge, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Ghodge 04

2013 Publication from Ghodge, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Godge

2013 Publication from Ghodge, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Ghodge 06

2013 Publication from Ghodge, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Ghodge 07

2013 Publication from Ghodge, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Ghodge 08

2013 Publication from Ghodge, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Ghodge 08

2013 Publication from Ghodge, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Ghodge 09

2013 Publication from Ghodge, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Ghodge 10

2013 Publication from Ghodge, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Ghodge 11

2013 Publication from Ghodge, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Ghodge 13

2013 Publication from Ghodge, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Almo 01

2013 Publication from Almo, et al. in Current Opinion in Structural Biology 23 Reprinted with permission. © Elsevier

2013 Almo 02

2013 Publication from Almo, et al. in Current Opinion in Structural Biology 23 Reprinted with permission. © Elsevier

2013 Almo 03

2013 Publication from Almo, et al. in Current Opinion in Structural Biology 23 Reprinted with permission. © Elsevier

2013 Almo 04

2013 Publication from Almo, et al. in Current Opinion in Structural Biology 23 Reprinted with permission. © Elsevier

2013 Daughtry 01

2013 Publication from Daughtry, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Daughtry 02

2013 Publication from Daughtry, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Daughtry 03

2013 Publication from Daughtry, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Daughtry 04

2013 Publication from Daughtry, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Daughtry 05

2013 Publication from Daughtry, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Daughtry 06

2013 Publication from Daughtry, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Daughtry 07

2013 Publication from Daughtry, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Daughtry 08

2013 Publication from Daughtry, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Daughtry 09

2013 Publication from Daughtry, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Pan 01

2013 Publication from Pan, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Pan 02

2013 Publication from Pan, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Pan 03

2013 Publication from Pan, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Pan 04

2013 Publication from Pan, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Pan 05

2013 Publication from Pan, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Pan 06

2013 Publication from Pan, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Pan 07

2013 Publication from Pan, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Pan 08

2013 Publication from Pan, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Pan 09

2013 Publication from Pan, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Pandya 01

2011 Publication from Schlessinger, et al. in Proc Natl Acad Sci USA Reprinted with permission. © National Academy of Sciences, USA

2013 Pandya 01

2013 Publication from Pandya, et al. in Proc Natl Acad Sci USA Reprinted with permission. © National Academy of Sciences, USA

2013 Pandya 02

2013 Publication from Pandya, et al. in Proc Natl Acad Sci USA Reprinted with permission. © National Academy of Sciences, USA

2013 Pandya 03

2013 Publication from Pandya, et al. in Proc Natl Acad Sci USA Reprinted with permission. © National Academy of Sciences, USA

2013 Pandya 04

2013 Publication from Pandya, et al. in Proc Natl Acad Sci USA Reprinted with permission. © National Academy of Sciences, USA

2013 Pandya 05

2013 Publication from Pandya, et al. in Proc Natl Acad Sci USA Reprinted with permission. © National Academy of Sciences, USA

2013 Pandya 06

2013 Publication from Pandya, et al. in Proc Natl Acad Sci USA Reprinted with permission. © National Academy of Sciences, USA

2013 Tian 01

2013 Publication from Tian, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Tian 02

2013 Publication from Tian, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Tian 03

2013 Publication from Tian, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Tian 04

2013 Publication from Tian, et al. in Biochemistry Reprinted with permission. © American Chemical Society

2013 Hitchcock 01

2013 Publication from Hitchcock, et al. in Journal of the American Chemical Society. Reprinted with permission. © American Chemical Society

2013 Hitchcock 02

2013 Publication from Hitchcock, et al. in Journal of the American Chemical Society. Reprinted with permission. © American Chemical Society

2013 Hitchcock 03

2013 Publication from Hitchcock, et al. in Journal of the American Chemical Society. Reprinted with permission. © American Chemical Society

2013 Hitchcock 04

2013 Publication from Hitchcock, et al. in Journal of the American Chemical Society. Reprinted with permission. © American Chemical Society

2013 Hitchcock 05

2013 Publication from Hitchcock, et al. in Journal of the American Chemical Society. Reprinted with permission. © American Chemical Society

2013 Hitchcock 06

2013 Publication from Hitchcock, et al. in Journal of the American Chemical Society. Reprinted with permission. © American Chemical Society

2013 Bryan 01

2013 Publication from Bryan, et al. Reprinted with permission. © International Union Crystallographyy

2013 Bryan 02

2013 Publication from Bryan, et al. Reprinted with permission. © International Union Crystallographyy

2013 Bryan 03

2013 Publication from Bryan, et al. Reprinted with permission. © International Union Crystallographyy

2013 Bryan 04

2013 Publication from Bryan, et al. Reprinted with permission. © International Union Crystallographyy

2013 Zhao 01

2013 Publication from Zhao, et al. Reprinted with permission. Copyright © Nature Publishing Group

2013 Zhao 02

2013 Publication from Zhao, et al. Reprinted with permission. Copyright © Nature Publishing Group

2013 Zhao 03

2013 Publication from Zhao, et al. Reprinted with permission. Copyright © Nature Publishing Group

2013 Zhao 04

2013 Publication from Zhao, et al. Reprinted with permission. Copyright © Nature Publishing Group

2013 Dong 01

2013 Publication from Dong, et al. Reprinted with permission Oxford University Press. Copyright © Bioinformatics

2013 Dong 02

2013 Publication from Dong, et al. Reprinted with permission Oxford University Press. Copyright © Bioinformatics

2013 Dong 03

2013 Publication from Dong, et al. Reprinted with permission Oxford University Press. Copyright © Bioinformatics

2013 Dong 04

2013 Publication from Dong, et al. Reprinted with permission Oxford University Press. Copyright © Bioinformatics

2013 Dong 05

2013 Publication from Dong, et al. Reprinted with permission Oxford University Press. Copyright © Bioinformatics

2013 Dong 06

2013 Publication from Dong, et al. Reprinted with permission Oxford University Press. Copyright © Bioinformatics

2013 Goble 01

2013 Publication from Goble, et al. Reprinted with permission. Copyright © American Chemical Society

2013 Goble 02

2013 Publication from Goble, et al. Reprinted with permission. Copyright © American Chemical Society

2013 Goble 03

2013 Publication from Goble, et al. Reprinted with permission. Copyright © American Chemical Society

2013 Goble 04

2013 Publication from Goble, et al. Reprinted with permission. Copyright © American Chemical Society

2013 Goble 05

2013 Publication from Goble, et al. Reprinted with permission. Copyright © American Chemical Society

2013 Goble 06

2013 Publication from Goble, et al. Reprinted with permission. Copyright © American Chemical Society